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KRT18/CK18/Cytokeratin 18 Antibody

KRT18/CK18/Cytokeratin 18 Antibody

Product Specifications

Product Name Alternative

KRT18, CK-18, Cytokeratin 18, Cytokeratin-18, K18, Keratin 18, CYK18, Keratin-18

Reactivity

Bovine, Equine, Guinea pig, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus

Immunogen

A synthetic peptide of human KRT18

Target

KRT18 / CK18 / Cytokeratin 18

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kD according to NP_000215.1) . Recommended concentration: 0.1-0.3 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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