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NEFM/NF-M Antibody

NEFM/NF-M Antibody

Product Specifications

Product Name Alternative

NEFM, 160 kDa neurofilament protein, NEF3, NF-M, NFM, Neurofilament 3

Reactivity

Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat

Immunogen

Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.

Target

NEFM / NF-M

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1 ug/ml.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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