Complement C3 Antibody
Complement C3 Antibody
Product Specifications
Product Name Alternative
C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3
Reactivity
Human, Monkey
Immunogen
Peptide with sequence QQRTFISPIKCRE, from the C-Terminus of protein sequence according to NP_000055.2.
Target
Complement C3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:128000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 125kD band in Human Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kD according to the largest fragment C3 alpha chain (aa672-1663) . Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kD band was successfully blocked by incubation with the immunizing peptide.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
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