Proliferation Marker IPO-38 Antibody
Proliferation Marker IPO-38 Antibody
Product Specifications
Reactivity
Human
Immunogen
A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
Target
Proliferation Marker IPO-38
Clonality
Monoclonal
Clone
IPO-38
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Concentration
1 mg/ml
Dilution
ELISA (1:10000 - 1:50000), IHC, IHC-Fr, IHC-P (10 μg/ml), WB (1:500 - 1:2000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: This antibody is suitable for ELISA, immunoprecipitation, western blotting and immunohistochemistry (frozen and formal in/paraffin) . The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-1 00 permeabilized) . An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide) . IPO-38 does not block the binding of the Ki-67 antibody. Tonsilar tissue is typically used as a positive control.
Tested Applications
ELISA, IHC, IHC-Fr, IHC-P, WB
Host or Source
Mouse
Preservative
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Isotype
IgM
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