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TNNT1/TNT Antibody

TNNT1/TNT Antibody

Product Specifications

Product Name Alternative

TNNT1, STNT, TNT, TNTS, Troponin-T1, skeletal, slow, ANM, Troponin T1, skeletal, slow, NEM5

Reactivity

Human

Immunogen

Peptide with sequence C-EKAQELSDWIHQLE, from the internal region of the protein sequence according to NP_003274.3NP_001119604.1NP_001119605.1.

Target

TNNT1 / TNT

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

0.5 mg/ml

Dilution

IHC, IHC-P (5 μg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 50kD band in Human Skeletal Muscle lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kD according to NP_003274.3. The 50kD band was successfully blocked by incubation with the immunizing peptide.

Tested Applications

ELISA, IHC, IHC-P, WB

Host or Source

Goat

Preservative

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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