EGFR Antibody
EGFR Antibody
Product Specifications
Product Name Alternative
EGFR, EGF Receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1
Reactivity
Human
Immunogen
Synthetic 16 amino acid peptide from C-Terminus of human EGFR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) ; Marmoset, Rat (94%) ; Mouse (88%) ; Bovine, Dog, Hamster, Elephant, Horse (81%) .
Target
EGFR
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Immunoaffinity purified
Concentration
0.8 mg/ml
Dilution
IHC, IHC-P (2.5 - 5 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 ug/ml.
Tested Applications
IHC, IHC-P
Host or Source
Rabbit
Preservative
PBS, 0.1% Sodium Azide
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