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FFAR2/GPR43 Antibody

FFAR2/GPR43 Antibody

Product Specifications

Product Name Alternative

FFAR2, FFA2R, Free fatty acid receptor 2, G protein-coupled receptor 43, G-protein coupled receptor 43, LSSIG, GPR43, Gpcr43

Reactivity

Human

Immunogen

Synthetic 16 amino acid peptide from C-Terminus of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%) ; Monkey (94%) .

Target

FFAR2 / GPR43

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Immunoaffinity purified

Concentration

1 mg/ml

Dilution

ELISA, IHC, IHC-P (20 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 14 ug/ml.

Tested Applications

ELISA, IHC, IHC-P

Host or Source

Rabbit

Preservative

PBS, 0.1% Sodium Azide

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