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GPCRW/GPR18 Antibody

GPCRW/GPR18 Antibody

Product Specifications

Product Name Alternative

GPR18, GPCRW, G protein-coupled receptor 18, NAGly receptor, G-protein coupled receptor 18, N-arachidonyl glycine receptor

Reactivity

Human

Immunogen

Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human GPR18. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) ; Marmoset, Bat, Elephant, Horse (94%) ; Bovine, Rabbit, Pig (89%) ; Mouse, Rat, Pufferfish (83%) .

Target

GPCRW / GPR18

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Immunoaffinity purified

Concentration

1 mg/ml

Dilution

ELISA, IHC, IHC-P (5 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.

Tested Applications

ELISA, IHC, IHC-P

Host or Source

Rabbit

Preservative

PBS, 0.1% Sodium Azide

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