TPRA1/GPR175 Antibody

Synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human TPRA1 / GPR175. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Pig, Platypus (100%) ; Monkey, Rabbit, Opossum, Turkey, Lizard, Zebrafish (94%) ; Chicken, Seabass, Salmon, Stickleback (88%) .

Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.