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TPRA1/GPR175 Antibody

TPRA1/GPR175 Antibody

Product Specifications

Product Name Alternative

TPRA1, G protein-coupled receptor 175, Transmembrane protein 227, TMEM227, TPRA40, GPR175

Reactivity

Bovine, Canine, Equine, Hamster, Human, Mammal, Mouse, Porcine, Rat

Immunogen

Synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human TPRA1 / GPR175. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Pig, Platypus (100%) ; Monkey, Rabbit, Opossum, Turkey, Lizard, Zebrafish (94%) ; Chicken, Seabass, Salmon, Stickleback (88%) .

Target

TPRA1 / GPR175

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Immunoaffinity purified

Concentration

1 mg/ml

Dilution

IHC, IHC-P (10 - 20 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.

Tested Applications

IHC, IHC-P

Host or Source

Rabbit

Preservative

PBS, 0.1% Sodium Azide

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