CHRM3/M3 Antibody
CHRM3/M3 Antibody
Product Specifications
Product Name Alternative
CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 receptor, EGBRS, HM3, M3 muscarinic receptor
Reactivity
Human, Monkey
Immunogen
Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) ; Mouse, Rat, Sheep, Bovine, Hamster, Elephant, Horse, Rabbit, Pig (95%) ; Dog, Opossum (90%) ; Lizard (85%) ; Panda, Guinea pig (80%) .
Target
CHRM3 / M3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Immunoaffinity purified
Concentration
1 mg/ml
Dilution
ELISA, IHC, IHC-P (10 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Tested Applications
ELISA, IHC, IHC-P
Host or Source
Rabbit
Preservative
PBS, 0.1% Sodium Azide
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