Anti-Brevican Antibody

Mouse Monoclonal Antibody specific to Brevican

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Brain-enriched hyaluronan-binding protein, BEHAB

Gene Name

Bcan

NCBI Gene ID

UniProt

P55068

Cellular Locus

[Isoform 1]: Secreted, extracellular space, extracellular matrix.; [Isoform 2]: Membrane; Lipid-anchor, GPI-anchor.

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 219-655 of rat Brevican (accession no. P55068).

Target Antigen

Brevican core protein

Target

Brevican

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Isoform 2 may function as a chondroitin sulfate-bearing cell surface receptor.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~140kDa is detected. Smaller cleavage products are also detected. <br><br>Positive control: Rat brain lysate. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat Brevican.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a145kDa core protein that bears up to three covalently-linked CS chains. It is also is secreted as a 145kDa core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, a 55kDa N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.