PNGase F

PNGase F, a glycosidase, catalyzes the cleavage of an internal glycoside bond in an oligosaccharide. PNGase F removes nearly all N-linked oligosaccharides from glycoproteins. PNGase F can release N-glycans from glycoproteins in glycoanalytical workflows[1][2].

Product Specifications

CAS Number

[83534-39-8]

UNSPSC

12352204

Hazard Statement

H334

Target

Biochemical Assay Reagents; Glycosidase

Type

Enzyme

Related Pathways

Metabolic Enzyme/Protease; Others

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/pngase-f.html

Solubility

H2O : ≥ 100 mg/mL

Smiles

[PNGaseF]

Precautions

H334

References & Citations

[1]Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31 (1) :2-7. |[2]Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87 (20) :10199-204.