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Rabbit HA Tag Antibody

HA Tag Antibody

Product Specifications

Target

HA Tag

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Antigen Affinity Purified

Concentration

1 mg/ml

Dilution

WB - 1:1,000 - 1:30,000; IP - 1 - 4 μg/mg lysate; ICC - 1:100 - 1:400; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Form

Liquid

Storage Conditions

2 - 8°C

Notes

For research use only.

Applications Notes

Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P) .Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Tested Applications

ELISA, ICC, IP, WB

Host or Source

Rabbit

Preservative

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide

Isotype

IgG

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