Rabbit V5 Tag Antibody
V5 Tag Antibody
Product Specifications
Target
V5 Tag
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Antigen Affinity Purified
Concentration
1 mg/ml
Dilution
WB - 1:1,000 - 1:30,000; IP - 1 - 4 μg/mg lysate; ICC - 1:100 - 1:400; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Form
Liquid
Storage Conditions
2 - 8°C
Notes
For research use only.
Applications Notes
Format: Whole IgG. Application Information: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P) .Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Tested Applications
ELISA, ICC, IP, WB
Host or Source
Rabbit
Preservative
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Isotype
IgG
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