Mycoplasma Staining Kit

BioVision's Mycoplasma Staining Kit provides an ultrasensitive, rapid and simple fluorescence based microscopic assay for the visual identification of mycoplasma infections in laboratory cell cultures. Mycoplasma infections are relatively common in laboratory cell cultures. It has been estimated that between 5% and 35% of all cell cultures are infected. Mycoplasmas have been shown to alter the growth rate of cells in culture, induce chromosomal aberrations, influence amino acid and nucleic acid metabolism and cause membrane aberrations. BioVision’s Mycoplasma Staining Kit contains a MycoFluor Reagent and Antifade Mounting Medium. The MycoFluor reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope with an excitation and emission maxima of 350-360 nm and 450-460 nm respectively. Mycoplasma staining with MycoFluor reagent appears as fine particulate or filamentous staining in the cytoplasm at 100X magnification. Nuclei of the cells are also brightly stained by this method and thereby act as endogenous positive control for the staining procedure.