Human Glypican 4 / GPC4 Protein, His Tag (MALS verified)
Human Glypican 4, His Tag (GP4-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Ala 19 - Ser 529 (Accession # O75487-1) .
Product Specifications
Background
Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Accession Number
O75487-1
Expression Region
Ala 19 - Ser 529
Host
HEK293
Target
Glypican 4
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 4, His Tag (Cat. No. GP4-H52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
59.5 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
250ug*4
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/GP4-H52H3.pdf
Species
Human
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