Human Glypican 4 / GPC4 Protein, His Tag (MALS verified)

Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

O75487-1

Expression Region

Ala 19 - Ser 529

Host

HEK293

Target

Glypican 4

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 4, His Tag (Cat. No. GP4-H52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).