Anti-Secretogranin II Antibody

Rabbit Polyclonal Antibody specific to Secretogranin II

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Chromogranin-C, Secretogranin II, SgII [Cleaved into: Secretoneurin

Gene Name

SCG2

NCBI Gene ID

UniProt

P13521

Cellular Locus

Secreted. Note=Neuroendocrine and endocrine secretory granules.

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Synthetic peptide derived from the N-terminus of mature SgII protein: QRNQLLGKEPDLRLENVQK (Accession no. AAA36607).

Target Antigen

Secretogranin-2

Target

Secretogranin II

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IP

Field of Research

Hormones/Proteins

Purification Method

Whole antiserum

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Whole Antiserum

Form

Liquid

Buffer

Whole Antiserum

Function

Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules. {PubMed:19357184}.

Additionnal Information

Immunoblotting: use diluted 1:2,500-1:10,000. A band of ~86kDa is detected. Although the predicted mw of SgII is ~71kDa, the highly acidic nature of SgII causes it to run at a higher mw in SDS-PAGE. Immunohistochemistry: use diluted 1:1,000. Immunoprecipitation: use diluted 1:100-1:500. Positive control: rat PC12 adrenal pheochromocytoma cells

Storage Conditions

This antibody is stable for at least one (1) year at -20°C to -70°C. Avoid repeated freeze- thaw cycles.

Specificity

This antibody recognizes human, rat, and squirrel monkey SgII.

Formulation

Whole antiserum

Target Background

Secretogranin II (SgII), encoded by the gene SCG2, is a member of the granin family of neuroendocrine secretory proteins. The granin family also includes chromogranin A (CgA), chromogranin B (CgB), and several other secretogranin-like proteins. Studies in rodents suggest that the full-length protein, SgII, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. SgII occurs in a variety of neoplasms arising from endocrine and neuroendocrine cells and can be a useful histological tumor marker for those cells.