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Rabbit LAG3 Recombinant Monoclonal Antibody

LAG3 Antibody

Product Specifications

Product Name Alternative

CD antigen CD223; CD223; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC

UniProt

P18627

Reactivity

Human

Immunogen

Between 475 and C-terminus

Target

LAG3

Clonality

Recombinant

Clone

BLR027F

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified

Concentration

500 μg/ml

Dilution

Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 μl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 12 μl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000

Purity

Recombinant antibody was purified from cell culture supernatant

Form

Liquid

Storage Conditions

2 - 8°C

Notes

For research use only.

Applications Notes

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Tested Applications

FC, ICC, IF, IHC, IP, WB

NCBI Accession Number

NP_002277.4

Host or Source

Rabbit

Preservative

Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide

Isotype

IgG

Available Sizes

Frequently Asked Questions

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