PPARG/PPAR Gamma Antibody
PPARG/PPAR Gamma Antibody
Product Specifications
Product Name Alternative
PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma
Reactivity
Canine, Human
Immunogen
Human PPAR1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE) . Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Cat, Dog, Elephant, Panda (100%) ; Marmoset, Mouse, Rat, Bat, Hamster, Rabbit, Pig, Opossum (95%) ; Sheep, Bovine, Goat, Horse (90%) ; Turkey, Chicken (85%) ; Platypus, Trachemys (80%) .
Target
PPARG / PPAR Gamma
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Immunoaffinity purified
Dilution
IHC, IHC-P (5 μg/ml), WB
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Rabbit
Preservative
TBS, 0.5 mg/ml rAlbumin, 0.02% Sodium Azide, 50% Glycerol
Isotype
IgG
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