AKT3 Antibody
AKT3 Antibody
Product Specifications
Product Name Alternative
AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2
Reactivity
Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat
Immunogen
A synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human AKT3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) ; Hamster (94%) ; Zebrafish (89%) .
Target
AKT3
Clonality
Monoclonal
Clone
66C1247.1
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G purified
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (10 μg/ml), WB (1 - 2 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.
Tested Applications
IHC, IHC-P, WB
Host or Source
Mouse
Preservative
PBS, 0.05% Sodium Azide, 0.05% rAlbumin
Isotype
IgG1
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