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GPR44/CRTH2 Antibody

GPR44/CRTH2 Antibody

Product Specifications

Product Name Alternative

PTGDR2, CD294, CRTH2, CD294 antigen, DP2, DL1R, G protein-coupled receptor 44, G-protein coupled receptor 44, HCRTH2, DP2 prostaglandin receptor, GPR44, Prostaglandin D2 receptor 2

Reactivity

Human

Immunogen

Synthetic 20 amino acid peptide from C-Terminus cytoplasmic domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%) ; Pig (85%) ; Elephant (80%) .

Target

GPR44 / CRTH2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Immunoaffinity purified

Concentration

1 mg/ml

Dilution

ELISA, IHC, IHC-P (15 μg/ml)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 15 ug/ml.

Tested Applications

ELISA, IHC, IHC-P

Host or Source

Rabbit

Preservative

PBS, 0.1% Sodium Azide

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