DMEM High Glucose

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts neurons glial cells HUVECs and smooth muscle cells as well as cell lines such as HeLa 293 Cos-7 and PC-12. Dulbecco's Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle's Minimal Essential Medium. The original DMEM formulation contained a low concentration of glucose (1 g/L) . However this DMEM formulation is available with higher glucose levels. DMEM also uses a sodium bicarbonate buffer system allowing for maintenance of physiological pH in a 5–10% CO2 environment. Importantly DMEM commonly requires fetal bovine serum (FBS) supplementation.