Anti-Acinus (IN) Antibody

Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Note=Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Peptide corresponding to aa 775- 789 of human AcinusL, aa 17-31 of human Acinus S and aa 48-62 of human AcinusS' which differ from those of mouse Acinus by one amino acid.

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells. {PubMed:10490026, PubMed:12665594, PubMed:18559500, PubMed:22203037, PubMed:22388736}.

Immunoblotting: use at 1-10ug/mL <br><br>Immunocytochemistry: use at 5-10ug/mL. <br><br>These are recommended concentrations. <br><br>Enduser should determine optimal concentrations for their applications. <br><br>Positive control: K562 cells or whole cell lysate.

A new inducer of chromatin condensation was recently identified and designated Acinus (for Apoptotic Chromatin Condensation Inducer in the Nucleus). Acinus is cleaved by caspase-3 and an additional protease to generate a small active peptide, p17, which causes chromatin condensation in vitro when it is added to purfied nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and are designated AcinusL, AcinusS, and AcinusS'.