Basic green 4

Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm[1][2][3].

[1]Chowdhury. S, et al. Sea shell powder as a new adsorbent to remove Basic Green 4 (Malachite Green) from aqueous solutions: Equilibrium, kinetic and thermodynamic studies. Chemical Engineering Journal, 164(1), 168–177. |[2]A A Baykov, et al. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay. Anal Biochem. 1988 Jun;171(2):266-70.|[3]Lloyd D. Wright, et al. Effect of Malachite Green and Formalin on the Survival of Largemouth Bass Eggs and Fry. The Progressive Fish-Culturist. 2011 Jan; Pages 155-157.