Goat anti-RSPO3 Antibody
Goat polyclonal antibody to RSPO3.
Product Specifications
Product Name Alternative
RSPO3; R-spondin 3 homolog (Xenopus laevis) ; CRISTIN1; FLJ14440; PWTSR; THSD2; R-spondin 3; thrombospondin, type I, domain containing 2; thrombospondin-like
Reactivity
Human, Mouse, Rat
Target
RSPO3
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SGYYGTRYPDINKC
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:128000.
Molecular Weight
30.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 37kDa band observed in lysates of cell lines HeLa, U2OS, A549 and KNRK, and in Mouse Brain lysates (calculated MW of 30.9kDa according to Human NP_116173.2, and 31.4kDa according to Mouse NP_082627.3, and Rat NP_001094460.1) . This molecular weigh. IHC: Paraffin embedded Human Colon. Recommended concentration: 4-6μg/ml. Positive staining was also seen in paraffin embedded Human Kidney at 4ug/ml.
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
84870
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