PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade

PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

Product Specifications

Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) 3 or20-mer (G4S) 4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains.

CAS Number

9007-83-4

Host

HEK293

Origin Species

Rabbit

Target

G4S linker

Conjugation

PE Labeled

Tag

Rabbit IgG | Rabbit Kappa

Applications

Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain) .

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution; 2-8°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.