4-Di-1-ASP

4-Di-1-ASP is a styryl dye used to stain glioma cells in living brain tissue for analysis of cell structure, viability, proliferation and endocytosis, cytokinesis and phagocytosis, as well as for observation of mitochondrial structures in living cells. 4-Di-1-ASP fluoresces green when imaged microscopically (λex /λem = 475/606 nm) [1][2].

Product Specifications

CAS Number

[959-81-9]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/4-di-1-asp.html

Purity

99.95

Solubility

DMSO : 43.75 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

C[N+]1=CC=C(/C=C/C2=CC=C(N(C)C)C=C2)C=C1.[I-]

Molecular Formula

C16H19IN2

Molecular Weight

366.24

Precautions

H315, H319, H335

References & Citations

[1]Lilia Y Kucheryavykh, et al. Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4- (4- (dimethylamino) -styryl) -N-methylpyridinium iodide (ASP+) . Biotechniques. 2012 Nov;53 (5) :305-9.|[2]Jensen J H Wong, et al. Simultaneous High-Throughput Conformational and Colloidal Stability Screening Using a Fluorescent Molecular Rotor Dye, 4- (4- (Dimethylamino) styryl) -N-Methylpyridinium Iodide (DASPMI) . J Biomol Screen. 2016 Sep;21 (8) :842-50.