Metronidazole ELISA Kit

Metronidazole is an antibacterial and antiprotozoal agent that belongs to the class of nitroimidazoles. It is used to treat parasitic infections such as trichomoniasis, giardiasis, gastrointestinal infections, and bacterial infections in humans. Although the exact mechanism is unknown, it is believed that an intermediate formed by the reduction of metronidazole by anaerobic organisms binds to the DNA and electron-transport proteins of these organisms and inhibits nucleic acid synthesis. The antibiotic has been demonstrated to cause cancer in mice and rats when exposed to high concentrations for a long period. Similar risks may be associated with humans. Due to its carcinogenic properties, this drug is prohibited from extra-label use in food animals. BioVision’s Metronidazole ELISA kit is used to quantitatively measure Metronidazole in muscle tissue, honey, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Metronidazole antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Metronidazole in the samples.