Fluo-3AM

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence[1].

Product Specifications

CAS Number

[121714-22-5]

Product Name Alternative

Fluo-3-pentaacetoxymethyl ester

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Fluo-3AM.html

Purity

99.0

Solubility

DMSO : 12.5 mg/mL (ultrasonic)

Smiles

ClC(C=C1C(C2=CC(OCCOC(C=C(C)C=C3)=C3N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C2)=C(C=C4Cl)C5=CC4=O)=C(C=C1O5)OCOC(C)=O

Molecular Formula

C51H50Cl2N2O23

Molecular Weight

1129.85

Precautions

H302, H315, H319, H335

References & Citations

[1]Loughrey, C. M., MacEachern, K. E., Cooper, J., & Smith, G. L. (2003) . Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium, 34 (1), 1–9. doi:10.1016/s0143-4160 (03) 00012-5