Mouse anti Green Fluorescent Protein (GFP)
Selected Variant
X1574M
Size: 100 µg
Product Specifications
Background
Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).
CAS Number
9007-83-4
UniProt
P42212
Host
Mouse
Isotype
IgG
Clone
F56-BA1.2.3
Conjugation
Unconjugated
Type
Monoclonal Antibody
Source
Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.
Applications
WB
Field of Research
Molecular Biology
Purification Method
Protein A/G Chromatography
Assay Principle
Antibody can be used for Western blotting (1-10 µg/mL). Optimal concentration should be evaluated by serial dilutions.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Prasher,D.C et.al. 'Primary structure of the Aequorea victoria green-fluorescentprotein.' Gene 111 (2), 229-233 (1992)_x000D_
2. Inouye,S. and Tsuji,F.I. 'Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein' FEBS Lett. 341 (2-3), 277-280 (1994)_x000D_
3. Cody,C.Wet.al. 'Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein' Biochemistry 32 (5), 1212-1218 (1993
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
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