Mouse anti Green Fluorescent Protein (GFP)

Product Specifications

Background

Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).

CAS Number

9007-83-4

UniProt

P42212

Host

Mouse

Isotype

IgG

Clone

F56-BA1.2.3

Conjugation

Unconjugated

Type

Monoclonal Antibody

Source

Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.

Applications

Field of Research

Molecular Biology

Purification Method

Protein A/G Chromatography

Assay Principle

Antibody can be used for Western blotting (1-10 µg/mL). Optimal concentration should be evaluated by serial dilutions.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1. Prasher,D.C et.al. 'Primary structure of the Aequorea victoria green-fluorescentprotein.' Gene 111 (2), 229-233 (1992)_x000D_ 2. Inouye,S. and Tsuji,F.I. 'Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein' FEBS Lett. 341 (2-3), 277-280 (1994)_x000D_ 3. Cody,C.Wet.al. 'Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein' Biochemistry 32 (5), 1212-1218 (1993