DNA Polymerase I Klenow Fragment from Escherichia coli

<strong>DNA Polymerase I Klenow Fragment from Escherichia coli</strong>_x000D_ <strong>Catalog number:</strong> B2016595_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 25 units_x000D_ <strong>Molecular Weight or Concentration:</strong> 103 kDa_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> DNA Polymerase I, Klenow Fragment from Escherichia coli_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Green MR, Sambrook J. E. coli DNA Polymerase I and the Klenow Fragment Cold Spring Harb Protoc. 2020 May 1;2020(5):100743._x000D_ 2: Ahlqvist J, Linares-Pastén JA, Jasilionis A, Welin M, Håkansson M, Svensson LA, Wang L, Watzlawick H, Ævarsson A, Friðjónsson ÓH, Hreggviðsson GÓ, Ketelsen Striberny B, Glomsaker E, Lanes O, Al-Karadaghi S, Nordberg Karlsson E. Crystal structure of DNA polymerase I from Thermus phage G20c Acta Crystallogr D Struct Biol. 2022 Nov 1;78(Pt 11):1384-1398._x000D_ 3: Saghatelyan A, Panosyan H, Trchounian A, Birkeland NK. Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1 Microbiologyopen. 2021 Jan;10(1):e1149._x000D_ 4: Yang Y, LiCata VJ. Pol I DNA polymerases stimulate DNA end-joining by Escherichia coli DNA ligase Biochem Biophys Res Commun. 2018 Feb 26;497(1):13-18._x000D_ 5: Bhardwaj A, Ghose D, Thakur KG, Dutta D. Escherichia coli β-clamp slows down DNA polymerase I dependent nick translation while accelerating ligation PLoS One. 2018 Jun 20;13(6):e0199559._x000D_ 6: Bailey MF, Van der Schans EJ, Millar DP. Dimerization of the Klenow fragment of Escherichia coli DNA polymerase I is linked to its mode of DNA binding Biochemistry. 2007 Jul 10;46(27):8085-99._x000D_ 7: Gao X, Gethers M, Han SP, Goddard WA 3rd, Sha R, Cunningham RP, Seeman NC. The PX Motif of DNA Binds Specifically to Escherichia coli DNA Polymerase I Biochemistry. 2019 Feb 12;58(6):575-581._x000D_ 8: Freemont PS, Ollis DL, Steitz TA, Joyce CM. A domain of the Klenow fragment of Escherichia_coli DNA polymerase I has polymerase but no exonuclease activity Proteins. 1986 Sep;1(1):66-73._x000D_ 9: Porecha R, Herschlag D. RNA radiolabeling Methods Enzymol. 2013;530:255-79._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/7035456">10: Brown WE, Stump KH, Kelley WS. Escherichia coli DNA polymerase I. Sequence characterization and secondary structure prediction J Biol Chem. 1982 Feb 25;257(4):1965-72.</a>_x000D_ _x000D_ <strong>Products Related to DNA Polymerase I Klenow Fragment from Escherichia coli can be found at</strong> <a href="https://moleculardepot.com/product-category/Enzymes/"> Enzymes</a>