Anti-High MW Kininogen Heavy Chain Antibody

Mouse Monoclonal Antibody specific to High MW Kininogen - Heavy Chain

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

α-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor [Cleaved into: Kininogen-1 heavy chain; T-kinin

Gene Name

KNG1

NCBI Gene ID

UniProt

P01042

Cellular Locus

Secreted, extracellular space.

Host

Mouse

Reactivity

Human

Immunogen

Purified human high molecular weight kininogen.

Target Antigen

Kininogen-1

Target

High MW Kininogen Heavy Chain

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, coagulation assays

Field of Research

Coagulation factors.

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

Additionnal Information

This antibody may be used in ELISA and Western blots to detect human HMWK light chain. It may also be used in coagulation assays. <br><br>Enduser should determine optimal antibody concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes the common heavy chain (~64kDa) of human HMWK and can neutralize the inhibitory activity of HMWK on platelet calpain.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

High molecular weight kininogen (HMWK) is a circulating plasma protein which participates in the initiation of blood coagulation, and in the generation of the vasodilator bradykinin via the Kallikrein-kinin system. HMWK is inactive until it either adheres to binding proteins beneath an endothelium disrupted by injury, thereby initiating coagulation, or it binds to intact endothelial cells or platelets for functions other than coagulation. HMWK is an alpha-globulin with six functional domains. It circulates as a single-chain 626 amino acid polypeptide . The heavy chain contains domains 1, 2, and 3; the light chain, domains 5 and 6. Domain 4 links the heavy and light chains.