Anti-PHAP III Antibody

Rabbit Polyclonal Antibody specific to PHAP III

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

LANP-like protein, LANP-L

Gene Name

ANP32E

Gene ID

81611

NCBI Gene ID

UniProt

Q9BTT0

Accession Number

NP_112182

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids near the carboxy terminus of human PHAP III (accession no. NP_112182)

Target Antigen

Acidic leucine-rich nuclear phosphoprotein 32 family member E

Target

PHAP III

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. {PubMed:24463511}.

Additionnal Information

Western Blot: Use at 1ug/ml. Positive control: A549 cell lysate. Immunohistochemistry: use at 2ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse and rat PHAP III (35kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The PHAP proteins (tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome- mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.