Hoechst 33342 (trihydrochloride)
Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1].
Product Specifications
CAS Number
[875756-97-1]
Product Name Alternative
BisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Autophagy
Type
Dye Reagents
Related Pathways
Autophagy
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Hoechst-33342-trihydrochloride.html
Purity
99.97
Solubility
DMSO : ≥ 46 mg/mL|H2O : ≥ 5.6 mg/mL
Smiles
[H]Cl.[H]Cl.[H]Cl.CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1
Molecular Formula
C27H31Cl3N6O
Molecular Weight
561.93
Precautions
H315, H319, H335
References & Citations
[1]Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011 (1) :pdb.prot5557.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Citation 01
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