Dengue NS1 Antibody
Mouse monoclonal antibody to Dengue NS1
Product Specifications
Reactivity
Virus
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Purification
Purified monoclonal IgG by protein A chromatography.
Purity
95% pure (12% SDS-PAGE and Coomassie staining) .
Form
2 vials of sterile Filtered clear colorless solution.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Applications: Lateral flow immunoassay. Assay Conditions: 1. 1-2 days fresh prepared gold particle with maximal homogeneity in gold particle size, λ 525-529, OD 1.2-1.4. 2. Conjugation at pH 8.5, conjugation time should be 6-8 hours. 3. Blocking condition: remove supernatant and re-suspend gold pellet bound with anti-dengue NS1 to OD 8.0- 8.2, add casein to the final 0.3-0.4%. 4. Treat sample pad with 50mM sodium borate, 0.5% mouse serum and 0.5% Triton X-100, pH8.0
Host or Source
Mouse
Preservative
Dengue NS1 Antibody solution for gold conjugation (3.6mg/ml) in PBS and Sodium nitrate. Dengue NS1 Antibody solution for membrane coating (2.2mg/ml) in PBS and Sodium nitrate.
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