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HSP27 Antibody

Mouse monoclonal antibody to HSP27

Product Specifications

Product Name Alternative

HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.

Reactivity

Human

Immunogen

Anti-human HSP27 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP27 amino acids 1-205 purified from E. coli

Clonality

Monoclonal

Clone

P2A5AT

Conjugation

Unconjugated

Purification

HSP27 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Form

Sterile Filtered colorless solution.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Applications: HSP27 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50

Host or Source

Mouse

Preservative

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Isotype

Mouse IgG1 heavy chain and κ light chain

Available Sizes

Frequently Asked Questions

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