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PKM2 Antibody

Mouse Monoclonal antibody to PKM2.Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence.

Product Specifications

Product Name Alternative

Pyruvate kinase isozymes M1/M2, EC 2.7.1.40, Pyruvate kinase muscle isozyme, Pyruvate kinase 2/3, Cytosolic thyroid hormone-binding protein, CTHBP, THBP1, M2PK, PKM2, PK3, PK2, PKM, TCB, OIP3, MGC3932, Tumor Type M2 Pyruvate Kinase.

Reactivity

Human

Immunogen

Anti-human PKM2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PKM2 amino acids 1-531 purified from E. coli

Clonality

Monoclonal

Clone

PAT1B10AT

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

PKM2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Applications: PKM2 antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results

Host or Source

Mouse

Preservative

1mg/ml containing PBS, pH-7.4, & 0.02% Sodium Azide and 10% glycerol.

Isotype

Mouse IgG1 heavy chain and κ light chain

Available Sizes

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