BrdU Antibody
Mouse monoclonal antibody to BrdU
Product Specifications
Immunogen
5-iodouridine covalently coupled to KLH
Clonality
Monoclonal
Clone
YR-BrdU
Conjugation
Unconjugated
Purification
Protein A column
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Applications: Immunohistochemistry, flow cytometry. Note: This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat. Titer: 1:500- 1:2,000 (assay dependent)
Host or Source
Mouse
Preservative
1mg/ml in PBS (after reconstitution) .
Isotype
Mouse IgG1
Available Sizes
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