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BrdU Antibody

Mouse monoclonal antibody to BrdU

Product Specifications

Immunogen

5-iodouridine covalently coupled to KLH

Clonality

Monoclonal

Clone

YR-BrdU

Conjugation

Unconjugated

Purification

Protein A column

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Applications: Immunohistochemistry, flow cytometry. Note: This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat. Titer: 1:500- 1:2,000 (assay dependent)

Host or Source

Mouse

Preservative

1mg/ml in PBS (after reconstitution) .

Isotype

Mouse IgG1

Available Sizes

Frequently Asked Questions

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