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TagRFP Antibody

Goat polyclonal antibody to TagRFP (orange fluorescent protein) . TagRFP is a basic (constitutively fluorescent) fluorescent protein, monomeric engineered derivate of fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It has very low acid sensitivity. TagRFP is a ~26 kDa protein that is optimally excited at a 555 nm and has a maximum of emission at 584 nm. This fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.

Product Specifications

Product Name Alternative

RFP antibody.

Immunogen

Antigen: Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli. Antigen Sequence: MASSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHR

Target

Red Fluorescent Protein

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Epitope affinity purified

Concentration

Please inquire

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 μl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The antibody solution should be gently mixed before use.

Tested Applications

IEM, IF, IHC-Fr, IHC-P, WB

Host or Source

Goat

Isotype

IgG

Frequently Asked Questions

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