Mouse anti Gram Negative Bacteria DNA Adenine Methylase

DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.

Antibody can be used for Western blotting (1-5 µg/mL). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions. The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Pogolotti, AL Jr., et al. 'On the mechanism of DNA-adenine methylase.' J Biol Chem, 1988, 263, 7461-7464._x000D_ 2. Heithoff, D.M., et al. ‘An essential role for DNA adenine methylation in bacterial virulence.’ Science, 284, 967-970