Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (HLR-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*33:03) & Ile 21 - Met 119 (B2M) & HVKITDFGR peptide (Accession # AAA79865.1 (HLA-A*33:03) & P61769-1 (B2M) & HVKITDFGR).

Product Specifications

Specifications

Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein is produced by co-expression of HLA and B2M loaded with EGF R peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.2 kDa and 11.7 kDa. The protein migrates as 39-45 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gly 25 - Ile 308 (HLA-A*33:03) & Ile 21 - Met 119 (B2M) & HVKITDFGR peptide

Host

HEK 293

Target

HLA-A*3303 & B2M & EGF R(HVKITDFGR)

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (Cat. No. HLR-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).