Anti-ESE-1 Antibody

Rabbit Polyclonal Antibody specific to ESE-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

E74-like factor 3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1, ESE-1

Gene Name

ELF3

NCBI Gene ID

UniProt

P78545

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

N-terminal sequence MAATCEISNIFSNYFS and C-terminal sequence SSGWKEEEVLQSRN of human ESE-1 protein.

Target Antigen

ETS-related transcription factor Elf-3

Target

ESE-1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity). {PubMed:10391676, PubMed:10644990, PubMed:10773884, PubMed:11036073, PubMed:11313868, PubMed:12414801, PubMed:12624109, PubMed:12682075, PubMed:12713734, PubMed:14715662, PubMed:14767472, PubMed:15075319, PubMed:15169914, PubMed:15794755, PubMed:16307850, PubMed:17060315, PubMed:9129154, PubMed:9234700, PubMed:9336459, PubMed:9395241, PubMed:9417054}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution <br><br>ELISA: use at 1:1,000-1:5,000 dilution.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human ESE-1 (39-41 kD).

Formulation

PBS.

Target Background

Epithelium-specific gene regulation plays a critical role during embryogenesis, and the epithelial cell lineage is the first differentiated cell type to appear after fertilization. Until recently, very few epithelial cell-restricted transcription factors had been described. Members of the ets transcription factor/oncogene family have been implicated in human carcinogenesis following the discovery of several distinct chromosomal translocations involving members of the ets family in different types of cancer. ets factors play key roles in transcriptional control of stringently regulated genes, such as genes involved in tissue development, differentiation, angiogenesis, cell cycle control and cell proliferation. ESE-1 is the first member of the ets family that was found to be exclusively expressed and inducible in epithelial cells.