LAG3 Detection Set

Product Specifications

Background

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

Host

Llama

Reactivity

Human

Immunogen

LAG3 antibodies were raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.

Clonality

Monoclonal

Conjugation

Unconjugated

Type

Detection Sets

Purification

LAG3 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein. They also contains a myc-tag for detection.

Concentration

Antibody 1 mg/mL

Buffer

LAG3 Antibodies are supplied in PBS.

Shipping Conditions

Dry Ice

Storage Conditions

LAG3 antibodies should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

Sdab

Background Reference 01

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.