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ER Signaling Protein Detection Set

Product Specifications

Background

For images please see PDF data sheet

Reactivity

Human

Immunogen

Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Clonality

Polyclonal

Type

Detection Sets

Purification

Antibodies are supplied as affinity chromatography purified IgG.

Concentration

Antibody 1 mg/mL

Buffer

PBS containing 0.02% sodium azide.

Shipping Conditions

Blue Ice

Storage Conditions

Stable at 4˚ C for three months, store at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=PSI-1814

Applications Notes

These polyclonal antibodies can be used for detection of ATF6, Caspase 12, IRE1p and XBP1 by immunoblot at 1 - 2 μ g/mL. ATF6, Caspase 12, IRE1p and XBP1 antibodies can detect their respective proteins via immunohistochemistry at 1 - 10 μ g/mL, and Immunoflourescence.

Background Reference 01

Kleizen B and Braakman I. Protein folding and quality control in the endoplasmic reticulum. Curr. Opin. Cell Biol. 2004; 16:343-9.

Background Reference 02

Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94) : functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

Background Reference 03

Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress. Methods 2005; 35:373-81.

Background Reference 04

Bertolotti A, Zhang Y, Hendershot LM, et al. Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response. Nat. Cell Biol. 2000; 2:326-32.

Tested Applications

WB, IHC, IF

Physical Properties

Liquid

Curated Selection

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