Breast Lysate
Product Specifications
Background
Breast tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μ g/ml of aprotinin, 5 μ g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Type
Lysates
Concentration
2 mg/mL
Buffer
Breast lysate is supplied in SDS sample buffer containing 50 mM DTT.
Shipping Conditions
Blue Ice
Storage Conditions
Store at 2-8˚ C for continuous use. For extended storage, freeze working aliquots at -70˚ C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=1311
Tested Applications
WB
Species
Human
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
