WT1 Peptide
Product Specifications
NCBI Gene ID
7490
Swiss Prot
P19544
Accession Number
NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3
Type
Peptides
Buffer
WT1 peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.
Shipping Conditions
Room Temp
Storage Conditions
Store at -20˚ C. When reconstituted minimize freezing and thawing.
Applications Notes
WT1 Peptide is used for blocking the activity of WT1 antibody (catalog number 46-601) . The WT1 antibody ships at 0.5 mg/mL and the WT1 Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
NCBI Official Name
Wilms tumor 1
NCBI Organism
Homo sapiens
Other Product Names
GUD, AWT1, WAGR, WT33, NPHS4, WIT-2, EWS-WT1, Wilms tumor protein
Tested Applications
Blocking
Species
Human
Protein ID
65507714
Physical Properties
Lyophilized
Frequently Asked Questions
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