PDE5A Peptide
Product Specifications
NCBI Gene ID
8654
Swiss Prot
O76074
Accession Number
NP_001074, NP_237223, NP_246273, (NP_236914)
Type
Peptides
Buffer
PDE5A peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.
Shipping Conditions
Room Temp
Storage Conditions
Store at -20˚ C. When reconstituted minimize freezing and thawing.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=46-153P
Applications Notes
PDE5A Peptide is used for blocking the activity of PDE5A antibody (catalog number 46-153) . The PDE5A antibody ships at 0.5 mg/mL and the PDE5A Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
NCBI Official Name
Phosphodiesterase 5A, cGMP-specific
NCBI Organism
Homo sapiens
Other Product Names
CN5A, PDE5, CGB-PDE, cGMP-binding cGMP-specific phosphodiesterase
Tested Applications
Blocking
Species
Human
Protein ID
61744435
Physical Properties
Lyophilized
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