HIWI Peptide
Product Specifications
NCBI Gene ID
9271
Swiss Prot
Q96J94
Accession Number
NP_004755.2
Type
Peptides
Buffer
HIWI peptide is supplied as 100 μ g of dried peptide. When the peptide is reconstituted in 200 μ L water, the concentration will be 0.5 mg/mL.
Shipping Conditions
Room Temp
Storage Conditions
Store at -20˚ C. When reconstituted minimize freezing and thawing.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=45-735P
Applications Notes
HIWI Peptide is used for blocking the activity of HIWI antibody (catalog number 45-735) . The HIWI antibody ships at 0.5 mg/mL and the HIWI Peptide ships as a 100 ug pellet. When peptide is reconstituted in 200 uL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
NCBI Official Name
Piwi-like RNA-mediated gene silencing 1
NCBI Organism
Homo sapiens
Other Product Names
HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1
Tested Applications
Blocking
Species
Human
Protein ID
66346725
Physical Properties
Lyophilized
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